Boss Ian Holloway neglected to name the quartet, who stayed out until the early hours of Sunday morning despite their involvement in Monday afternoon's reserve clash with Rochdale. It is understood none of the players were in the starting line-up for last weekend's 3-0 win over npower Championship leaders Southampton.
Club rules state players should not be out late 48 hours before a game and those concerned were ordered to train on their own at the Seasiders Squires Gate training base on Wednesday and are to be left behind when their team-mates head to Vicarge Road
Holloway told the Blackpool Gazette: "I am very proud of my lads. They have been brilliant this season and it is important they understand that. But I am only proud when they behave the right way. When they don't, it lets myself, the team and the fans down.
"Last weekend, some of them were playing on Monday but they were out on the Saturday till God knows what hour in the morning. That is unacceptable, so I have spoken about that to the whole squad.
"For me, Monday's game was absolutely vital. Maybe they thought differently because it wasn't a first-team match. But it is about a game, and how dare you disrespect the game and not prepare properly for it?"