Enoch Showunmi and Andy Robinson are set to return to Tranmere's squad for the trip to Oldham.
The forward duo are back in full training following respective leg and knee problems and played 90 minutes against Manchester City reserves on Monday night.
Showunmi's injury has kept him away from first-team duties since the turn of the year, while Robinson last played in mid-December.
Rovers manager Les Parry told the club's official website: "Both played another 90 minutes on Monday for the reserves. They both showed great attitude.
"They didn't just get through the game, they put in a lot of hard work and I was made up to see that. So they are both in contention for Saturday."
Stoke striker Ryan Brunt's loan spell expires after the game, while defenders David Raven (groin) and Zoumana Bakayogo (shin) remain sidelined.