World Cup 2010 - Germany suffer Cacau blow
The Brazilian-born frontman suffered a strain of the abdominal muscle during training.
"He will definitely not play," said team manager Oliver Bierhoff.
"They will join the squad for the last training before the match," said Bierhoff.
Schweinsteiger picked up a thigh muscle injury in their final Group D match against the Black Stars and missed two days of training.
Defender Boateng was also doubtful following a calf muscle injury in the same game.
Coach Joachim Loew said on Friday that 20-year-old midfielder Toni Kroos, who came on as a substitute for Schweinsteiger late in the Ghana match, could replace him from the start.
Left-back Holger Badstuber, who had started in Germany's two other Group D encounters against Australia and Serbia, or Marcell Jansen would replace Boateng if the new Manchester City player was ruled out.