Rolland set a time of 1:53.30 to top the timesheets ahead of Regina Sterz and Maria Hoefl-Riesch.
On a day where few of the skiers pushed themselves to their limits, Lindsey Vonn finished in ninth place after going fastest in the first session.
The American - a triple winner at Lake Louise last year and holder of 11 victories at the venue that has become known as 'Lake Lindsey' - stood up well before the finish.
The third training run is scheduled for Thursday, with the races to come on Friday, Saturday and Sunday.
Top 10 results:
1 ROLLAND Marion FRA 1:53.30
2 STERZ Regina AUT 1:53.34
3 HOEFL-RIESCH Maria GER 1:53.36
4 WEIRATHER Tina LIE 1:53.56
5 FENNINGER Anna AUT 1:53.57
6 MERIGHETTI Daniela ITA 1:53.76
7 GOERGL Elisabeth AUT 1:53.79
8 MAZE Tina SLO 1:53.87
9 VONN Lindsey USA 1:54.23
10 COOK Stacey USA 1:54.33
- Sports & Recreation
- Lindsey Vonn