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Gunners fans have protested for KSE, who are owned by Stan Kroenke, to sell the club after they tried to join the failed European Super League earlier this month.
The debacle has reopened old wounds between supporters and Kroenke, sparking interest from Ek.
The Swedish billionaire has joined forces with Arsenal legends Dennis Bergkamp, Patrick Vieira and Thierry Henry - and he is ready to make a formal offer.
But now KSE have released a statement, insisting they have no plans to sell and their ambition is to help Arsenal “win the biggest trophies in the game”.
A statement on Tuesday evening read: “In recent days we have noted media speculation regarding a potential takeover bid for Arsenal Football Club.
“We remain 100% committed to Arsenal and are not selling any stake in the Club. We have not received any offer and we will not entertain any offer.
“Our ambition for Arsenal remains to compete to win the biggest trophies in the game and our focus remains on improving our competitiveness on the pitch to achieve this.
“Stan Kroenke and Josh Kroenke.
“Kroenke Sports & Entertainment.”