Bolt got off to a decent start and took control of heat one by the 40-metre mark before cruising across the finish line in 10.00 seconds.
Asafa Powell, who had previously failed to complete a competitive race all season due to hamstring problems, enters the semi-finals with the second fastest time after winning heat four in 10.02 seconds.
"Happy to complete my first race in a year," Powell, who had been battling hamstring problems, told Reuters after completing his first competitive race of the season. "It was quite easy."
World champion Yohan Blake, who has a wild-card entry to the August 10-18 world championships in Moscow, is not competing in the Jamaican trials.
The other top qualifiers are Kemar Bailey-Cole, who recovered from a poor star to take heat two in 10.04, while Sheldon Mitchell won heat three in 10.11.
The Jamaican women, minus Olympic champion Shelly-Ann Fraser-Pryce, who has a wild-card entry, will begin competing for spots on Jamaica's team to Moscow on Friday.
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