Ons Jabeur became the latest big-name casualty at the Australian Open when she suffered a second-round defeat to Marketa Vondrousova.
Jabeur has been hampered by knee and back injuries at the start of the season and the second seed suffered more pain on Rod Laver Arena, where the excellent Vondrousova sealed a 6-1 5-7 6-1 win in the early hours of Friday morning in Melbourne.
Vondrousova has been troubled by multiple wrist injuries since she was a runner-up at the French Open in 2019, but appears to have put those issues behind her.
The Czech left-hander dominated the first and final sets after Jabeur showed her fighting spirit in the second to force a decider at Melbourne Park.
A runner-up at Wimbledon and the US Open last year, tenacious Tunisian Jabeur appeared to be in some pain and struggling for breath during a match in which she made 50 unforced errors.
The world number two struck 27 winners to her opponent's 17, but followed the likes of Rafael Nadal, Casper Ruud and Emma Raducanu in making early exits when she overcooked a forehand.
Vondrousova, ranked 78th after an injury-hit 2022 season in which missed three of the four grand slams, will face compatriot Linda Fruhvirtova in round three.