The 31-year-old opened her World Championship campaign as part of the Norwegian mixed relay team that stormed to gold against a Martin Fourcade anchored French quartet.
She had to settle for silver as she returned to the Czech Republic course for the 7.5km sprint though as Olena Pidhrushna took a historic gold medal for Ukraine ahead of the Norwegian.
But there was to be no denying Berger in the 10km pursuit as she recovered from two poor shoots to produce a flawless fourth that set her on her way to victory by almost 19 seconds.
Poland’s Krystyna Palka didn't have the legs to catch Berger as she finished 18.5seconds behind in second while Pidhrushna added bronze to her win in the sprint in Nove Mesto.
With five events down Pidhrushna victory in the sprint is the only event Norway haven't won with Emil Hegle Svendsen winning the men's sprint and pursuit.
Berger, starting six seconds behind Pidhrushna, quickly took a narrow lead over the Ukrainian with both still neck and neck after shooting clear the first time into the range.
However Pidhrushna surged ahead after repeating the trick on her second trip with Berger falling behind after missing a target before failing to hit two third time around.
Pidhrushna also missed two as the lead completely changed hands with Olga Zaitseva leading and Palka just behind however things changed after the fourth and final shoot.
While Zaitseva and Palka missed one, Berger went clear and, having trailed by 23.4 at one stage, would not be caught over the final leg as she produced a winning time of 28:48.4minutes.
Palka held on to silver while for Pidhrushna shooting clear fourth time into the range proved significant as she overtook Zaitseva to take bronze exactly three seconds behind her Polish rival.
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- Olena Pidhrushna