Manchester United defender Daley Blind has been omitted from the final selection for Ronald Koeman's first Netherlands squad.
The 28-year-old was included in a 33-man preliminary squad for international friendlies against England and Portugal this month.
But Blind, who has not played for United since January 5 due to an ankle injury, was among those cut from the final group.
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PSV's Luuk de Jong and Wolfsburg's Jeffrey Bruma have also been left out by Koeman, who handed maiden call-ups to a number of young players including Ajax's teenage forward Justin Kluivert - son of Netherlands icon Patrick.
Marco Bizot, Hans Hateboer, Guus Til and Wout Weghorst are also in the Netherlands squad for the first time.
Netherlands squad: Marco Bizot (AZ), Jasper Cillessen (Barcelona), Jeroen Zoet (PSV); Nathan Ake (Bournemouth), Matthijs de Ligt (Ajax), Stefan de Vrij (Lazio), Timothy Fosu-Mensah (Crystal Palace), Hans Hateboer (Atalanta), Daryl Janmaat (Watford), Kenny Tete (Lyon), Patrick van Aanholt (Crystal Palace), Virgil van Dijk (Liverpool); Marten de Roon (Atalanta), Davy Propper (Brighton and Hove Albion), Kevin Strootman (Roma), Guus Til (AZ), Donny van de Beek (Ajax), Tonny Vilhena (Feyenoord), Georginio Wijnaldum (Liverpool); Ryan Babel (Besiktas), Steven Berghuis (Feyenoord), Memphis Depay (Lyon), Bas Dost (Sporting CP), Justin Kluivert (Ajax), Quincy Promes (Spartak Moscow), Wout Weghorst (AZ).