Chris Robshaw has been confirmed as captain of a squad that also features the return of experienced internationals Danny Care and James Haskell.
Care was excluded from England's RBS 6 Nations campaign on disciplinary grounds, while Haskell was unavailable because he was playing in the southern hemisphere.
The inclusion of Leicester's Tom Youngs as one of four hookers is the most left-field selection, given he has never started a Premiership match in that position. The converted centre has made 12 bench appearances for Leicester and will compete with first choice Dylan Hartley, Lee Mears and the uncapped Harlequin Joe Gray for the hooker's jersey.
Youngs will join his scrum-half brother Ben in the senior England squad for the first time and he is joined by Tigers team-mate Graham Kitchener as a fellow England newcomer. The Wasps pair of wing Christian Wade and flanker Joe Launchbury have been rewarded for their excellent Premiership seasons in a struggling side with a first senior call-up.
Lancaster has drafted in Bath flanker Carl Fearns and Exeter loose forward Tom Johnson after losing Tom Croft and Tom Wood to injury and Callum Clark to suspension.
Despite England's back-row selection issues, there is no return for Nick Easter or Toulon's Steffon Armitage, but Haskell is picked ahead of his return to Wasps from the Otago Highlanders next season.
Harlequins, who finished top of the regular season Premiership table and face Northampton at home in Saturday's semi-final, also provide lock George Robson and centre George Lowe. Saracens full-back Alex Goode and London Irish centre Jonathan Joseph also win their first senior England call-ups.
Mouritz Botha (Saracens)
Dan Cole (Leicester Tigers)
Alex Corbisiero (London Irish)
Paul Doran Jones (Northampton Saints)
Phil Dowson (Northampton Saints)
Carl Fearns (Bath Rugby)
Joe Gray (Harlequins)
Dylan Hartley (Northampton Saints)
James Haskell (Otago Highlanders)
Tom Johnson (Exeter Chiefs)
Graham Kitchener (Leicester Tigers)
Joe Launchbury (London Wasps)
Joe Marler (Harlequins)
Lee Mears (Bath Rugby)
Ben Morgan (Scarlets)
Matt Mullan (Worcester Warriors)
Tom Palmer (Stade Francais)
Geoff Parling (Leicester Tigers)
Chris Robshaw (capt, Harlequins)
George Robson (Harlequins)
Matt Stevens (Saracens)
Thomas Waldrom (Leicester Tigers)
Tom Youngs (Leicester Tigers)
Anthony Allen (Leicester Tigers)
Chris Ashton (Northampton Saints)
Brad Barritt (Saracens)
Mike Brown (Harlequins)
Danny Care (Harlequins)
Lee Dickson (Northampton Saints)
Owen Farrell (Saracens)
Toby Flood (Leicester Tigers)
Ben Foden (Northampton Saints)
Alex Goode (Saracens)
Charlie Hodgson (Saracens)
Jonathan Joseph (London Irish)
George Lowe (Harlequins)
Ugo Monye (Harlequins)
David Strettle (Saracens)
Manu Tuilagi (Leicester Tigers)
Jordan Turner-Hall (Harlequins)
Christian Wade (London Wasps)
Ben Youngs (Leicester Tigers)