Brian Kelly has received his flu vaccinations and returned to Gold Coast Titans training, while team-mate Bryce Cartwright has been granted an exemption on medical grounds.
Kelly and Cartwright were stood down by the Titans having refused injections ahead of the NRL season restarting later this month following its suspension in March due to the coronavirus pandemic.
The Queensland government had taken the stance that players who did not have the vaccine on non-medical grounds would not be permitted to train or play in the state.
The Titans imposed a deadline for Thursday for the players to either have the jabs or risk losing their contracts.
Gold Coast confirmed Kelly had returned to training having been vaccinated, but Cartwright had avoided the jabs due to medical reasons.
A statement from the Titans read: "Brian Kelly has received both the influenza vaccine and pneumococcal vaccinations and returned to training today in preparation for the NRL competition restart on 28 May.
"Bryce Cartwright provided the club with medical documentation seeking an exemption from the influenza vaccine protocol.
"This was subsequently passed on to Queensland's chief medical officer Jeannette Young for review.
"Dr. Young has granted Cartwright an exemption based on medical grounds.
"Cartwright is now eligible to return to training with the playing squad in preparation for the season restart."
Through two rounds of the 2020 NRL season, the Titans have still yet to win an NRL game having been beaten by Canberra Raiders and Parramatta Eels.