A joint statement by Trott's employers at the England and Wales Cricket Board and Warwickshire on Friday confirmed that he will stop playing with immediate effect.
Trott, who had to leave England's Ashes tour after the first Test in Brisbane last November for the same reason, made a short-lived comeback at the start of this season.
He played first in a friendly for Warwickshire against Gloucestershire at Edgbaston, then a first-class match against MCCU Oxford University and last week in his county's opening championship fixture against Sussex.
It was after that match, in which he scored 37 and 26, that he decided he could not continue.
The ECB and Warwickshire statement said: "At the completion of the LV=CC match between Warwickshire and Sussex on April 16, 2014 - in which he played - Jonathan experienced a repeat of stress-related symptoms which were diagnosed during England's Ashes series in Australia in the winter.
"Jonathan will now undergo further treatment to assist with his long-term rehabilitation.
"Both parties confirm that their primary concerns are for Jonathan's health and well-being, and reiterate that they will continue to offer him their full support."
Trott himself, 33 next week, added: "It was with the best intentions and hope that I returned to cricket with Warwickshire this month.
"Much to my disappointment, since my first game, I have felt the same anxieties that occurred during my time in Australia.
"It is not fair on my team-mates or myself to continue when I am unable to function at 100 per cent. In order to play at the highest level you have to be at 100 per cent and be able to concentrate fully at all times. Sadly I am unable to do this.
"With this in mind I have decided it is best for all involved that I continue my recovery on the sidelines for the time being.
"I would like to thank Warwickshire and the ECB for their continued support through this time."
- Sports & Recreation