As well as representing their most significant test to date on the road to the tournament in Qatar next year, the clash at Wembley is also the Three Lions’ last competitive outing before this summer’s rescheduled European Championship.
England’s stars will no doubt be eager to impress to ensure they are able to book a spot in Gareth Southgate’s final tournament squad.
Here, Standard Sport’s Dan Kilpatrick examines three key issues for England against Poland...
Kane's role under scrutiny
Harry Kane's habit of dropping into midfield came in for strange scrutiny from ITV pundits Ian Wright and Roy Keane during the win over Albania on Sunday, forcing Southgate to back his captain to keep roaming.
With the manager considering starting two speedy wingers in Raheem Sterling and Jesse Lingard on Wednesday, Kane's service from midfield could prove crucial again after he bagged another assist in Tirana.
England face defensive test
Even without Robert Lewandowski, Poland possess two strikers with top-level European experience in Krzysztof PiÄ tek and Arkadiusz Milik.
In response, England could switch to a back three and Piatek and Milik should provide the centre-backs with a stern physical examination, particularly John Stones, who is yet to truly be tested since his return to the squad.
Southgate's full-back question
Whether he plays a back three or four, Southgate has fierce competition at full-back. Luke Shaw is pressuring Ben Chilwell, while Reece James, Kyle Walker and Kieran Trippier are battling it out at right-back.
Wednesday’s selections should be revealing as to Southgate's preferred pair, with the manager having said he already knows his possible XI for the Euros.