Bouffier had hoped to take part in the all-asphalt event in his Peugeot 207 Super 2000, but finance problems have forced him to pull out.
The Frenchman will be back in action for the next round of the championship in Poland from 13-15 September.
Missing the rally is a major blow for Bouffier, who has been hoping to upgrade his car to a more powerful Peugeot 207 S2000: the Czech Rally is considered one of Europe’s most challenging rallies due to the proliferation of high-speed blasts through forests, sections of broken tarmac, a high likelihood of intermittent showers and the bumpy nature of some roads
The rally attracts thousands of fans each year, with the action beginning with a super special astage through the streets of Zlín on Friday 30 August.
There’s also a night stage on Saturday ahead of the traditional Sunday afternoon finish.
Starts: 17:00hrs, Friday 30 August, Náměstí Míru Square, Zlín
Finishes: 16:00hrs, Sunday 1 September, Náměstí Míru Square, Zlín
Entries received: 102
Stage distance: 234.78 kilometres (130.92 kilometres leg one, 103.86 kilometres leg two)
2012 winners: Juho Hänninen (FIN)/Mikko Markkula (FIN) ŠKODA Fabia Super 2000