Van Dijk, who has made 34 appearances for Southampton this season, has not played since he sustained the injury in Southampton's victory over Leicester City in January.
The 25-year-old, who was expected to return within two to three months, has been strongly linked with moves to Manchester City and Chelsea by British media.
"It's a long injury. We will see if Van Dijk can come back before the end of the season," Puel told reporters on Thursday.
Striker Manolo Gabbiadini, who has scored six goals for the club since joining in January, will miss Saturday's Premier League clash with Bournemouth as he recovers from a groin injury suffered during a 2-1 loss to Tottenham Hotspur earlier in March.
"I think that Gabbiadini cannot play this game. He is not available, but we will see for the next one. For the other players, I hope and think it's ok," Puel said.
Read the full article on eurosport.co.uk: Southampton's Puel hopeful Van Dijk will play again this season