Spurs will move into the national stadium for the 2017-18 campaign while work on their new 61,000-seater ground is completed.
It means Sunday's Premier League match at home to Arsenal will be the last north London derby at White Hart Lane and Manchester United will be their final opponents there on May 14.
Tottenham, who have played at White Hart Lane since 1899, last month extended the deadline for a final decision on Wembley but triggered the option already agreed with the Football Association on Friday.
The club were concerned building work would not be finished on time for the new stadium to open in August 2018, but Spurs have now given the switch the green light.
Chairman Daniel Levy said: "The Lane means a huge amount to each and every one of us and we needed to gain greater certainty on the delivery of the new stadium before we made the final decision to commence with the decommissioning of our iconic, historic home for some 118 years.
Read the full article on eurosport.co.uk: Tottenham confirm Wembley move for 2017/18 season