The 38-year-old American struck a perfect seven-iron from 179 yards. His ball landed on the green, ran up a slope and then took a hard turn to the left and trickled back down into the left side of the cup to wild cheers from the gallery.
The first ace in this year's Masters took Kuchar to five under par, the score he ended on as he finished with straight pars.
After celebrating wildly with his caddie on the tee, Kuchar walked to the green and retrieved his ball, which he signed and then presented to a young boy wearing a bright orange T-shirt in the front row of the crowd.
Speaking afterwards Kuchar said "One of the cool parts of our job is to bring a smile to kid's faces. For me that golf ball is not a keepsake but it could brighten up his day and maybe we'll see him back here playing in a couple of years."
Read the original article on Eurosport: Kuchar aces 16th hole at The Masters