England face Ukraine in Kiev on Tuesday with a one-point lead at the top of Group H but defeat would leave their hopes of automatic qualification for next year's eagerly-anticipated World Cup finals hanging by a thread.
Lampard, who began his international career against Belgium in 1999, said the milestone would be something to cherish.
"I will be very proud if I get on the pitch because it's been a long time but it wouldn't be enjoyable to reach 100 and get the wrong result," told a news conference.
"We are well aware that this is going to be a pivotal game. If we win we have it very much in our hands, although we still have two difficult home games to come."
Lampard missed Euro 2012 through injury and when he appeared to slip down the pecking order at Chelsea his international future looked uncertain.
He started against Moldova on Friday, however, and was influential in setting up England's opening goal scored by Gerrard.
"I know this won't go on forever," said Chelsea's record goalscorer who has notched 29 for England.
"But the carrot of Brazil, a huge footballing nation, a World Cup that everyone wants to be at, I would love to take part in that, it's as simple as that."
Lampard, who does not expect to be given the captain's armband for the night, is one of three centurions in the squad with Gerrard and Ashley Cole both on 104 caps.
His midfield combination with Gerrard has not always been a natural fit with critics suggesting they do not play well together, but Lampard believes England now have the perfect blend.
"I've always enjoyed playing with Steven," Lampard said. "We can talk all day about his qualities. But we had a nice mixture the other day, Steven can start a bit deeper and we can switch it around and with Jack Wilshere we have a world-class talent.
"It was an honour to play with them on Friday but Tuesday is going to be a lot tougher."
Ukraine started their qualifying campaign slowly but a 4-0 win in Montenegro and a 9-0 hammering of San Marino mean they are breathing down England's necks in the battle to win the group.
"I've been very impressed with them, I think they had a bad start to the group but against us they played with a lot of talent, technical ability and a lot of pace," Lampard said.
"We weren't at out best (in the home 1-1 draw at Wembley) that night but to not lose when you're not at your best is a good thing. We have to go there knowing they are a good team but need to believe in our own ability."
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- Steven Gerrard
- Jack Wilshere