Roberto Mancini's side may be struggling to regain their Premier League title this season, but their use of the social media platform was held up as an example of best practice.
Lewis Wiltshire, head of sport for Twitter UK, told the Soccerex European Forum in Manchester: "Manchester City have really started to innovate on Twitter."
Wiltshire said there were 11 sports teams in the world with more than a million followers, of which nine were football clubs. City have more than 786,000 followers.
"The challenge is how close can the club get to its fans," he said.
City captain Vincent Kompany was described by Wiltshire as one the best examples of a footballer using Twitter.
The defender used his official account - @VincentKompany - to ask for inspirational messages for the team which were displayed on the dressing room wall.
Kompany has more than 730,000 followers on Twitter. There are 10 million active Twitter users in the UK, two million of which are football fans.
- Sports & Recreation