Striker Bothroyd has missed the last six games due to an ankle injury while midfielder Semedo has been sidelined with a badly-cut knee.
Wednesday extended their unbeaten run to four matches with their goalless derby draw at Huddersfield on Saturday.
After a fourth straight clean sheet, Jones is unlikely to make any changes to his back four of Lewis Buxton, Anthony Gardner, Miguel Llera and Reda Johnson.
Jeremy Helan is available despite receiving two yellow cards at Huddersfield.
The on-loan Manchester City midfielder was shown a second yellow card midway through the first half but referee Mick Russell did not realise he had booked the same player for diving earlier in the game.
Gary Madine returned to the side on Saturday and is likely to continue but fellow forwards Chris O'Grady and Mamady Sidibe, on loan from Stoke, plus winger Jermaine Johnson are all pushing for recalls.
- Sports & Recreation
- Lewis Buxton
- Miguel Llera