On-loan pair Assombalonga and Mkandawire both missed the original 1-1 draw between the two sides having been denied permission by parent clubs Watford and Millwall to feature.
And despite returning for the last two games, the duo must again watch from the stands as the Shrimpers return to FA Cup action.
Defender Mark Phillips missed Saturday's goalless draw at Fleetwood due to a knee injury and again sits out, while manager Paul Sturrock is also waiting on other unnamed players who came away at the weekend nursing knocks, with late assessments due to be made.
But there is some good news for Southend with John Spicer and David Martin available after illness and fellow midfielder Michael Timlin also returns having been suspended against The Cod Army.
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