The on-loan right-back picked up a hamstring injury early in the second half of last Saturday's 1-1 draw with Crewe at Gresty Road and now faces around four weeks on the sidelines.
Jermaine Grandison remains another week or so away from his return having suffered a slight setback in his recovery from a hip injury, meaning Connor Goldson or Rob Purdie are the likely contenders to step in for Gayle.
Midfielder Mark Wright is closing in on his comeback from lengthy knee trouble but is not yet ready while defender Rob Edwards (thigh) continues to miss out.
Town parted ways with forward Luke Rodgers by mutual consent on Thursday, meaning he will not be involved.
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