Gayle has scored seven goals in nine appearances for Posh since arriving on loan from Dagenham & Redbridge but the 22-year-old is cup-tied for the third-round tie having played in the Daggers' first-round defeat to Bournemouth at the start of November.
Boss Darren Ferguson will be forced to make two further changes to the team that started the npower Championship win over Barnsley on New Year's Day.
Gavin Tomlin was allowed to play in that game despite being sent off in the defeat at Bristol City as Posh appealed against the red card. However, the appeal has failed and the forward will now begin a three-match suspension.
Defender Gaby Zakuani has since left to link up with the DR Congo squad ahead of their African Nations' Cup campaign.
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