The highly-rated Guidetti, 21, joined City at the age of 16 but his progress at the club has been hampered by illness and injuries.
Guidetti has appeared twice on the City bench this season but the club feel he would benefit from having greater opportunities elsewhere.
He is behind the likes of Sergio Aguero, Alvaro Negredo, Edin Dzeko and Stevan Jovetic at the Etihad Stadium.
Hughes told the club's official website: “We wanted to try and add different options to our attacking threat and John will certainly do that.
“He’s someone who if you get him in the right areas will make the most of opportunities that come his way because he’s a natural goal-scorer.
“There were several clubs who were interested in taking him on loan and we’re delighted that he has chosen to join us.”
Guidetti spent time on loan at Burnley just over three years ago and also had a successful spell at Feyenoord in the 2011-12 season.
A statement on City's website confirmed the deal at Stoke would run until June 30. The deal was made possible after Stoke confirmed they had converted midfielder Stephen Ireland's loan move from Aston Villa into a permanent deal.
That freed up another loan space for Guidetti to move to the Britannia Stadium. The Potters have Liverpool's Oussama Assaidi as their other permitted Premier League loanee.
- Sports & Recreation
- Alvaro Negredo
- Stevan Jovetic
- Sergio Aguero