Wigan midfielder Kiernan, Fulham striker Trotta and Watford defender Hodson have all been denied permission to play in the competition by their parent clubs.
Kiernan had to go off at half-time during Saturday's 2-1 win at Notts County with concussion so was unlikely to be fit anyway.
On-loan Norwich midfielder Tom Adeyemi, fit again after a month out with a hamstring injury, replaced Kiernan at Meadow Lane and he is eligible to play.
Boss Uwe Rosler gave back-up goalkeeper Richard Lee a run-out in the original tie, which ended 1-1, so he should figure again.
Midfielder Kevin O'Connor (ankle) and striker Farid El Alagui (knee) are still out.
- Sports & Recreation