Brazilian full-back Marcelo was stretchered out of Los Blancos' 3-1 win over Real Betis on Saturday evening with a hamstring injury.
AS have claimed that the defender will miss the next three weeks, meaning he will be unavailable for both legs of the last four tie with the Bundesliga side.
There is also concern over Luka Modric, who complained of a calf problem yesterday, and may be out for the next 10 days.
"The injuries were the worst aspect of the game," Real assistant coach Aitor Karanka told the post-match news conference.
"Marcelo felt a sharp pain. Modric's problem seems only to be a strain, but he said he felt something and we'll do some checks."
Goalkeeper Diego Lopez and striker Karim Benzema also seemed to pick up knocks during the encounter.
Lopez was unable to take the goalkicks near the end, and was holding his hip, while Benzema was replaced by Gonzalo Higuain after having complained of a kick to his ankle, though Karanka said neither injury seemed particularly serious.
Real fielded a weakened team against Betis and have made it clear their priority for the remainder of the season is the pursuit of tenth European Cup triumph, rather than trying to overhaul runaway La Liga leaders Barcelona.
Jose Mourinho's side travel to play Borussia Dortmund on Wednesday.
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