The Bees' unbeaten record came to an end in spectacular fashion as they had goalkeeper David Button sent off in a 4-0 defeat at Valley Parade.
But boss Rosler expects Tranmere to be on the end of a backlash when Brentford head north on Saturday.
"We have to take the defeat with grace and with our heads up," he said.
"The red card was a harsh decision but after that we are not blaming anyone else, we are looking at ourselves.
"We had a good talk at half-time and after the game. We will react this week in training and we are building up for Tranmere."
- Sports & Recreation
- David Button