Lester missed out with a back problem in midweek as former manager John Sheridan returned to the Proact Stadium with Plymouth and won 2-1 to deal a severe blow to the Spireites' hopes of reaching the play-offs.
Blues boss Paul Cook delivered a scathing attack on his team following the defeat on Tuesday night, accusing them of failing to turn up for what was the biggest game of the season so far.
Substitutes Alex Henshall, Armand Gnanduillet and Sam Hird will be hoping to start after they helped to improve what had been a dire performance against Argyle.
Danny Whitaker should also be in the frame for a recall after missing that game due to family reasons while fellow midfielder Craig Clay and striker Scott Boden are also options for Cook.
Chesterfield need to win on Saturday and beat Exeter at home on the final day of the season and hope Bradford get no more than two points from their remaining two matches if they are to gatecrash the play-offs.
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