The Three Lions captain limped out of the stadium and had his ankle strapped after a heavy challenge by Iran’s Morteza Pouraliganji in the second half of the 6-2 Group B win in Doha on Monday afternoon.
The scan is seen as precautionary rather than cause for alarm.
But there are doubts over Kane’s fitness after he originally came off in the 75th minute of that opening match, to be replaced by Callum Wilson.
England boss Gareth Southgate was confident after the game that Kane was okay, telling reporters: “I think Harry’s fine. It looked like a bad tackle but he carried on in the game.
“We took him off because we felt it was a moment in the game we could do that.”
Kane remains England’s most influential player as he chases his country’s all-time scoring record, with three goals needed to surpass Wayne Rooney.
Newcastle’s Wilson - who did gym work on Tuesday as the rest of England’s non-starters against Iran trained - and Marcus Rashford are alternative options to step into his place against the USA.
England Training Session for FIFA World Cup 2022 Qatar | 22nd November 2022
James Maddison remained absent from training on Tuesday, while Harry Maguire has been dealing with illness after being forced off against Iran.