Akdam and Stack The Deck lost their lives to add to the death of Our Conor on the opening day of the four-day event.
Akdam fractured a foreleg in the Handicap Hurdle in an incident that also left jockey Bryan Cooper, riding another horse Clarcam, with a broken arm.
Clarcam was not badly injured in the incident.
Stack The Deck was pulled up lame in the Champion Bumper with a fractured left foreknee.
On Monday, Our Conor, who fell during the Champion Hurdle, had to be put down due to a serious back injury.
The deaths put more unwanted scrutiny on the festival that has felt the wrath of animal rights protestors in the past.
The festival was roundly criticised in 2012 when five horses died in the space of two days. Matuhi was the sole death at the festival last year, but four more horses lost their lives at Cheltenham at other events at the course in 2013.
Various safety measures were introduced at Cheltenham after nine horses died at the 2006 meeting.
- Death & Funeral
- Sports & Recreation