Paul Lambert’s men were on the losing side as Anthony Knockaert’s brace handed them their 18th defeat of the season.
Ikeme has missed Wolves’ last three games owing to a hamstring injury he sustained while on international duty with Nigeria.
It proved a huge blow for the already much-depleted Molineux giants, whose dream of grabbing an English Premier League ticket has faded in thin air.
"We started really well and for half an hour we were really in the game. But we were lost before half-time, where a catalogue of errors lead to their goal,’’ Lambert said after the game.
‘’We didn't test their goalkeeper enough while they were clinical. They played really well, got men behind the ball and, in the end, they deserve to go up."
With this result, Brighton need just four points from their remaining four games to seal promotion to the Premier League for the first time in their history.
Wolves are 16th on the log with 51 points from 41 games , They travel to Leeds United for their next game on Monday.