Ciro Immobile joined Italy's injury list ahead of Friday's Nations League encounter with England.
The Lazio striker has suffered a muscle injury and was not included in the Azzurri's squad list for the game at San Siro.
Midfielder Sandro Tonali left the European champions' training camp on the day of the match as he has not recovered from an injury.
Coach Roberto Mancini will also be without the likes of Marco Verratti, Matteo Politano, Lorenzo Pellegrini and Davide Calabria.
Italy will remain in contention for the Nations League Finals if they come out on top in a repeat of last year's Euro 2020 final.
England, bottom of Group 3, will be relegated from League A if they are beaten in Milan.