Roberto Mancini’s Euro 2020 winners are back in competitive action on home soil as the San Siro hosts the first of two semi-finals.
The two last met in the last four of the continental competition as Italy edged out Luis Enrique’s side on penalties to advance to the final.
Italy will be aiming to preserve their remarkable 37-game unbeaten run, while Spain are missing several key players.
The winner will set up a meeting with either France or Belgium in Milan on Sunday.
Where to watch Italy vs Spain
TV channel: Today’s match will be televised live on Sky Sports Football, with coverage beginning at 7:30pm BST ahead of a 7:45pm kick-off.
Live stream: Sky Sports subscribers can watch the game live via the Sky Sports app or website.
Live coverage: Standard Sport will bring you all the match action, analysis and reaction in our live blog.
Read our dedicated match preview here.