The FA has upheld his three-match suspension for striking Wigan's Shaun Maloney during last Saturday's Premier League fixture.
An FA statement read: "Chelsea defender Branislav Ivanovic’s appeal against an FA charge for violent conduct has been dismissed.
"The incident, which was not seen by the match officials but caught on video, occurred during his side’s game against Wigan at Stamford Bridge on Saturday 7 April 2012.
"A Regulatory Commission heard the claim today and Ivanovic’s three match suspension will commence with immediate effect."
Liverpool also failed with their appeal against the red card issued to goalkeeper Alexander Doni in Tuesday's victory at Blackburn.
The Reds appealed citing wrongful dismissal but it was rejected by a Football Association regulatory commission, who ruled the one-match suspension remains.
It means third-choice goalkeeper Brad Jones is likely to play in goal in Saturday's FA Cup semi-final against Everton at Wembley, with first-choice Jose Reina already suspended.
Doni was sent off during the Premier League match for denying an obvious goalscoring opportunity.
Referee Anthony Taylor awarded a penalty to Rovers for the infringement only for substitute goalkeeper Jones to save Ayegbeni Yakubu's spot-kick.
Jones' appearance at Ewood Park was his first since December 2010 and only his third in 20 months since arriving from Middlesbrough.