Irving, who has now played in 32 of the Nets' 43 games this season, is averaging 25.6 points, 4.8 rebounds and 4.8 assists per game, and he has already eclipsed the 29 games he managed in the 2021-22 campaign.
The Nets were already without superstar Kevin Durant due to his knee injury, and were left even more shorthanded when Irving was withdrawn in the hours before tip-off against the Spurs.
In his absence, Brooklyn suffered their third loss in a row as no Nets players were able to reach 20 points.
Meanwhile, 23-year-old Nets center Nic Claxton became the youngest player since 22-year-old Rudy Gobert in 2015 to block at least three shots in nine consecutive games.
In comments before the game, head coach Jacque Vaughn called the injury "day to day".
"[Irving] just reported a little tightness," Vaughn said. "It's essentially just day to day. We'll check on him [Wednesday], and hopefully this is short-term."
The Nets are hopeful Irving will be able to return when they travel to take on the Phoenix Suns on Thursday.