A hip problem saw Little substituted just before half-time against Shrewsbury on Saturday and the full-back will undergo a late fitness test. However, midfielder Grant McCann is not expected to recover from his slight groin injury.
Winger Nathaniel Mendez-Laing, who scored the winning goal in Posh's 1-0 victory over the Shrews, is pushing for a starting place against the side where he spent a short period on loan last season while Lee Tomlin could be included among the substitutes after making good progress in his recovery from an ankle injury.
Darren Ferguson's side go into the game on the back of six straight Sky Bet League One wins and four clean sheets and are just one point adrift of table-topping Leyton Orient.
Sheffield United caretaker boss Chris Morgan could name an unchanged starting line-up at Peterborough.
Morgan has no new injury or suspension problems after the Blades climbed off the bottom of the table with their second win of the season against Port Vale on Saturday.
Striker Jose Baxter remains doubtful due to a thigh injury, but defender Tony McMahon (thigh) returned to play the full 90 minutes at the weekend and midfielder Florent Cuvelier (leg) was back on the bench.
Striker Shaun Miller made his first appearance since last Boxing Day against Port Vale after recovering from a serious knee injury and is expected to be among the substitutes again.
- Sports & Recreation