Matt Smith turned from hero to villain for Oldham as Chesterfield began the defence of the Johnstone's Paint Trophy with a 2-1 victory over the team they beat in last season's Northern Area final.
Smith gave Oldham a first-half lead but scored an own goal in added time after Danny Whitaker had equalised and then seen a penalty saved by Dean Bouzanis.
Jennison Myrie-Williams netted a second-half double to send Port Vale into the second round with a comfortable 2-0 victory against disappointing Tranmere.
Coventry's maiden foray in the competition ended with shootout success as they beat Burton 10-9 on penalties following a 0-0 draw.
Brave York withstood almost constant Rotherham pressure to snatch a smash-and-grab 1-0 victory with a late winner from winger Matty Blair.
Notts County secured their first win in the competition for 11 years as striker Enoch Showunmi scored his first goal on his first start in a 2-1 victory over Scunthorpe.
New-boy Bobby Grant scored his first goal since moving to Rochdale and then netted the crucial penalty in the shootout to send his side through 4-2 on penalties after a 2-2 draw against Fleetwood. James McGuire scored both goals for the Cod Army in normal time but missed his spot-kick.
Jack Redshaw and Kevin Ellison took Morecambe through to the second round with a 2-0 win over Lancashire rivals Accrington.
Preston best Carlisle 3-1 on penalties after the sides had fought out a 1-1 draw in normal time.