It will mark the first time the Wanderers midfielder has attended a game since he suffered a cardiac arrest in an FA Cup tie between Bolton and Spurs at White Hart Lane on March 17.
Bolton manager Owen Coyle said: "We are absolutely delighted that we will be able to welcome Fabrice and his family back to the Reebok.
"It's fitting that the match is between these two clubs and that Fabrice is able to come along.
"I know Fabrice is very keen to show his appreciation to both sets of fans for their support."
Midfielder Muamba, 24, came out of hospital two weeks ago.
"It feels good to be back doing normal stuff again," Muamba said on this Twitter account on Tuesday.
The match is of huge importance to both teams.
Bolton are in 18th place, level on 34 points with Queens Park Rangers who are outside the relegation zone thanks to a superior goal difference. Wigan Athletic and Aston Villa sit above them on 37 points.
Tottenham, along with Newcastle United and Chelsea who clash on Wednesday in London, are challenging for fourth place which brings a Champions League qualifying round berth.
Spurs are fourth on 62 points after 35 games, level with Newcastle and a point ahead of Chelsea.