Henoc Mukendi booked Northampton's place in the next round of the Johnstone's Paint Trophy after they came from behind to beat 10-man Colchester 2-1.
Northampton's best chance fell to Adebayo Akinfenwa who saw his header hit the bar before Freddie Sears broke the deadlock after half an hour, superbly volleying home a right wing cross from ex-Cobbler Henderson.
But Akinfenwa equalised six minutes into the second half from the penalty spot after Roofe was upended in the box.
And three minutes later Mukendi opened his account when he converted a cross from the impressive Ishmel Demontagnac.
Super-sub Sam Saunders came off the bench to earn Brentford their second win in four days over Crawley as he scored the only goal in a 1-0 win.
Argentine midfielder Andres Gurrieri scored a superb 30th-minute winner as Plymouth beat Aldershot 2-1 after Paris Cowan-Hall and Craig Reid had earlier traded goals.
Moses Odubajo's first-half strike proved enough to hand Leyton Orient a 1-0 victory over winless Barnet.
Goals from Ryan Cresswell and Britt Assombalonga handed Southend United a 2-0 win over Dagenham & Redbridge at Roots Hall.
Oxford twice hit back from behind to progress with a 4-2 win at Cheltenham.
Wycombe's Dean Morgan bagged a brace to send League One opponents Portsmouth crashing out with a 3-1 victory at Fratton Park.
- Adebayo Akinfenwa
- Freddie Sears