Quagliarella showed superb technique to finally break the resistance of the visitors just past the hour and added a second goal five minutes later when he rolled the ball past Chievo keeper Stefano Sorrentino after another superb piece of skill.
The rejuvenated striker, who scored the equaliser in the 2-2 draw at Chelsea in the Champions League in midweek, put on an impressive display that gave Antonio Conte's side the cutting edge up front that they have missed so far this season.
The win puts Juventus on 12 points from four games ahead of Napoli and Lazio, who will bid to match the leaders when they visit Catania and host Genoa respectively on Sunday.
Despite starting without Andrea Pirlo, Stefan Lichtsteiner and Sebastian Giovinco, Juve dominated from the start and thought they had scored when Mirko Vucinic tapped in Mauricio Isla's shot after 26 minutes but it was rightly ruled offside.
Chievo's impressive Sorrentino then had to make smart saves from Emanuele Giaccherini on the half hour and Kwadwo Asamoah three minutes later as Juventus piled on the pressure.
Giorgio Chiellini should have given the champions the lead in the 62nd minute when he was left free in the six-yard box at a corner but he somehow headed straight at Sorrentino.
But Quagliarella, making his first start of the season, got the reward his effort deserved from the resulting corner.
The striker opened the scoring with a spectacular scissors-kick from near the penalty spot in the 64th minute and doubled his tally five minutes later when he instinctively lifted the ball over Dario Dainelli and beat the exposed Sorrentino.
"This has been perhaps the best week of my career, to score three goals in two games in not easy to do," said Quagliarella. "For me tonight was the game of my life... Juve haven't beaten Chievo for four years and I'm happy for the three points."
- Stefano Sorrentino