They are two sides who know this competition well - Portugal won the inaugural Nations League in 2019 ,while local rivals Spain finished as runners-up in the second edition two years later.
This time they’re both in Group A2 along with Switzerland and the Czech Republic as the teams face a hectic period of four games in a 10-day spell.
However, with a World Cup to come at the end of the year, every match is crucial as teams look to ensure they finalise their preparations ahead of the trip to Qatar.
Date, kick-off time and venue
Spain vs Portugal is scheduled for a 7.45pm BST kick-off time tonight, Thursday June 2, 2022.
The Estadio Benito Villamarin in Seville will host the match.
Where to watch Spain vs Portugal
TV channel: Spain vs Portugal will be shown live on Premier Sports 2. It currently costs £9.99 for a monthly subscription in the UK.
Live stream: Premier Sports subscribers will also be able to watch the game online via the Premier Player on mobile and tablet devices.
Live blog: You can also follow all the action with updates from Standard Sport’s live blog this evening.
Spain vs Portugal team news
Spain XI: Simon; Azpilicueta, D. Llorente, Torres, Alba; Soler, Busquets, Gavi; Torres, Morata, Sarabia.
Subs: Raya, Sanchez, Carvajal, Martinez, Garcia, Alonso, M. Llorente, Rodri, Olmo, Asensio, Fati, De Tomas.
Portugal XI: Costa; Cancelo, Danilo, Pepe, Guerreiro; Otavio, Moutinho, Fernandes, B. Silva; Leao, A. Silva.
Subs: Patricio, R. Silva, Carmo, Duarte, Dalot, Mendes, Palhinha, Nunes, Neves, Vitinha, Carvalho, Ronaldo, Jota, Guedes, Horta.
Spain vs Portugal prediction
For two sides with so much attacking talent, the most recent meetings have not been particularly entertaining.
It’s finished goalless the last two times the teams have played each other, thought there was that classic 3-3 draw at the 2018 World Cup.
With plenty of tired legs at the end of a long club season, and the Nations League not a massive priority at this stage, it’s probably reasonable to expect a more low-key affair this time.
Head to head (h2) history and results
Spain wins: 17
Portugal wins: 6