Brownlee won Olympic gold ahead of Spaniard Javier Gomez and younger brother Jonathan at London 2012 last summer but the three have managed to avoid each other this season.
Alistair missed the Madrid leg with injury but won in Kitzbuhel last time out, which saw Jonathan pull out with a stomach bug, while Gomez has been consistent throughout.
However all three are set to line up together in Hamburg for the sixth round of the World Series and Alistair admits the race could go either way given the season that’s been so far.
"I think it's the first time we've all raced since the Olympics but we are obviously all coming in at slightly different stages of our year," said Alistair.
"Javier has done pretty much every race under the sun in the last six months. Jonny hasn't done very many races and neither have I.
"We'll be racing in very different ways. Jonny might have the slight advantage at sprint distance, he’s got that speed."
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