Chambers was freed to compete at London 2012, pending qualification after the Court of Arbitration for Sport ruled his lifetime Olympic ban, imposed by the British Olympic Association, contravened the World Anti-Doping Agency's code.
Since returning to the sport, the former world indoor champion has been having to scratch out a living at minor events, with many leading promoters refusing to invite them to their meetings.
However, he will line-up against American Walter Dix, last year's IAAF World Championships 100 metres and 200m silver medallist, Trinidad's Richard Thompson, the 2008 Olympic 100m runner-up, and fellow Briton Marlon Devonish in Manchester.
Olympic champion Usain Bolt ran a world best 14.35 sec over the rarely ran 150m straight distance three years ago.
Dix believes he is in shape to attack the USA record of 14.51 sec that Tyson Gay achieved in Manchester a year ago.
Meanwhile, Chambers, currently training in Jamaica, could challenge the British best performance of 14.87 sec.