Although the first leg ended in a 0-0 draw, the Foxes will feel that they had several chances to notch an away goal on the night and, back on home soil, the feeling is that a good showing should be enough to see off their opposition and extend an unbeaten home record in Europe to 10 matches - a run which stretches back to the 1997/98 UEFA Cup.
Leicester have been in good form of late, beating Aston Villa at the weekend to maintain their push for a Champions League spot through the Premier League. Brendan Rodgers is likely to stick with a strong line-up as they look to go deep into the competition.
READ MORE: Champions League knock-out fixtures by date and time
Slavia only managed a draw domestically at the weekend, though remain top and unbeaten in league play this season, with an eight-point gap down to second-place local rivals Sparta.
Here’s everything you need to know ahead of the deciding leg of the clash.
When is the match?
The game kicks off at 8pm on Thursday, 25 February.
Where can I watch it?
The match will be shown live on BT Sport ESPN. Subscribers can stream the game via the BT Sport app and desktop website.
What is the team news?
Leicester remain without defenders Wes Morgan and Wesley Fofana, while James Justin is now out for the season. James Maddison and Ayoze Perez are injuries in the attacking line, while Kelechi Iheanacho is suspended. Dennis Praet is also out.
Slavia will miss David Hovorka and Petr Sevcik, while Tomas Holes and Stanislav Tecl both need fitness tests ahead of the game.
LEI - Schmeichel; Amartey, Evans, Soyuncu, Thomas; Ndidi, Tielemans; Albrighton, Under, Barnes; Vardy
SVP - Kolar; Bah, Kudela, Zima, Boril; Hromada, Provod; Sima, Stanciu, Olayinka; Kuchta
Leicester - 11/18
Draw - 31/10
Slavia Prague - 6/1
Even with a number of key players injured, the Foxes have the form and attacking options to get through this 90 minutes with a win. Leicester 2-0 Slavia Prague.